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. 2015 Jan 7;8:7. doi: 10.1186/s13071-014-0605-4

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© Rodriguez-Valle et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Semi-quantitative analysis of the expression of rms transcripts in R. microplus tick samples . A and C: PCR products obtained from cDNA samples from different tick’ tissues. The PCR products were run in 1% Tris Borate agarose gels. B and D: Normalised mRNA density was obtained after the densitogram analyses of the amplified PCR products. All experiments were conducted in triplicate. Data were represented as the mean ± standard deviation (SD). The symbols ** and *** indicate statistical significance with p < 0.05 and p < 0.001, respectively.