Table 1.
Various sorbents used in protein SPE.
| SPE type | Material | Usage |
|
| ||
| Reversed-phase | C3, C4, C8 | Proteins |
| C18 | Peptides | |
|
| ||
| Normal phase | ZIC-HILIC | Glycoproteins, glycopeptides |
| TSKgel Amide-80 | Proteins | |
| Waters HILIC | Proteins, peptides | |
| Cotton* | Glycopeptides, glycans after release from peptides | |
|
| ||
| IEX | WCX, SCX | Proteins |
| WAX, SAX | Proteins | |
|
| ||
| Metal-chelating | Ti, Fe | Phosphopeptides |
| Ga | Phosphopeptides | |
| Cu | (Phospho)peptides | |
|
| ||
| Affinity | Lectin | Glycans, glycopeptides |
| Boronic acid | Glycans, glycopeptides | |
| Blue dye | Albumin | |
| Protein A/G | Immunoglobulins | |
| Heparin | IgG | |
| RNA/DNA | Plasmids, DNA binding proteins | |
| Purine/pyrimidine derivatives | For example, ATP/GTP using enzymes | |
| Coenzymes | Coenzyme-dependent enzymes | |
| Vitamins | Vitamin binding proteins | |
| Antibodies | Proteins, peptides | |