Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 2;74(23):7090–7102. doi: 10.1158/0008-5472.CAN-14-0305

Figure 2. BET protein Inhibitor JQ1 reduces c-Myc expression and attenuates primary MCC cell proliferation.

Figure 2

A. Decreased c-Myc expression in MCC-3 and MCC-5 treated with JQ1 (800nM) for 72 hours by qRT-PCR and immunoblotting. The mRNA expression of target genes was normalized to that of MRPS2 and a value of 1.0 was assigned to the mRNA expression of target genes in the control group (means ± SEM), (**P<0.01 vs control); β-actin was used as a loading control for immunoblotting. B. MCC-3 and MCC-5 cells are sensitive to JQ1 inhibition. MCC cell lines (MCC-3 and MCC-5) were cultured with JQ1 at different concentrations (200nM, 400nM and 800nM) for 24, 48, 72 and 120 hours respectively (means ± SEM), (*P<0.05, **P<0.01, ***P<0.001 vs control). C. Decreased colony formation in MCC-3 and MCC-5 cells treated with JQ1 (800nM) compared with that of DMSO control. A total of 3000 cells were mixed with methylcellulose medium with JQ1 (800nM) or DMSO and the mixture was plated in each 35-mm dish for 21 days at 37 °C (means ± SEM), (***P<0.001 vs control); scale bars = 200µm.