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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 2;74(23):7090–7102. doi: 10.1158/0008-5472.CAN-14-0305

Figure 3. JQ1 induced cell cycle arrest is via upregulation of p21, p27 and p57 in both MCC cell lines.

Figure 3

A. Representative FACS histograms show the distribution of MCC cells in cell cycle after JQ1 treatment. MCC-3 and MCC-5 cells were treated with JQ1 (800nM) for 72 hours and stained with BrdU and 7-AAD followed by flow cytometry analysis (means ± SEM). Bar graphs show the percentage of MCC cells in each cell cycle phase. Data presented as means ± SEM of triple experiments. B. Expressions of cell cycle associated genes in MCC cells (MCC-3 and MCC-5) treated with JQ1 (800nM) for 72 hours by qPCR and immunoblotting. The mRNA expression of target genes was normalized to that of MRPS2 and a value of 1.0 was assigned to the mRNA expression of target genes in the control group (means ± SEM), (***P<0.001 vs control); β-actin was used as a loading control.