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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 2;74(23):7090–7102. doi: 10.1158/0008-5472.CAN-14-0305

Figure 5. c-Myc knockdown recapitulates cell cycle arrest in MCC cells.

Figure 5

A–B. Successful knockdown of c-Myc expression in transfected cells (MCC-3 and MCC-5) by qRT-PCR and immunoblotting. The mRNA expression of target genes was normalized to that of MRPS2 and a value of 1.0 was assigned to the mRNA expression of target genes in the control group (means ± SEM), (***P<0.001 vs Luc shRNA); β-actin was used as a loading control for immunoblotting. C-D. Representative FACS histograms show the distribution of c-Myc knockdown or control MCC cells in cell cycle after JQ1 (800nM) treatment for 72 hours (means ± SEM).