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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Neurobiol Dis. 2014 Dec 3;74:180–193. doi: 10.1016/j.nbd.2014.11.015

Figure 4. Western blot analysis of endogenous Parkin localization in cortical neurons after glutamate exposure.

Figure 4

Non-transfected cortical neurons were treated and harvested on DIV15 for cell fractionation. Western blot analyses were completed for Parkin levels and respective loading controls on the following cellular fractions: (A) whole cell, (B) mitochondria crude enrichment, (C) endoplasmic reticulum-enriched, and (D) mitochondria-associated membrane-enriched (MAM-enriched). Note that in the MAM-enriched fraction (D), presence of both FACL4 and calreticulin was assessed to verify presence of MAM. FACL4 was used as the loading control. (E) Quantitative results from Western blot analyses presented as the difference of the mean, calculated as treatment minus respective control (n= 4–5; * = p<0.05, +/− 95% C.I.).