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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: Cancer Res. 2013 Aug 16;73(19):5905–5913. doi: 10.1158/0008-5472.CAN-13-1511

Figure 2. Colonic mitotic activity & polyp load are exacerbated in IL-10-deficiency.

Figure 2

(A) Upper histogram depicts quantification of Ki67 staining within healthy appearing tissue of the colon B6, CD4CreIL-10fl/fl, IL-10−/−, APCΔ468, CD4CreIL-10fl/flAPCΔ468, and IL-10−/−xAPCΔ468 mice. Data represent the ratio of the height of Ki67-staining cells/total height of each villi per 200X view. Lower histogram (mean ± SEM) depicts colon length of mice in millimeters (mm).

(B) Representative Ki67 staining of colons. Black bar represents average height of Ki67+ cells within the villi.

(C) Colonic polyp counts of the various mouse strains.

(D) Photographs of distal colon segments from APCΔ468, CD4CreIL-10fl/flAPCΔ468, and IL-10−/− mice.

(E) Upper panels show representative 100X colonic polyp from an APCΔ468, CD4CreIL-10fl/flAPCΔ468, and IL-10−/−xAPCΔ468 mice. Double-headed arrows depict width of expanded stromal regions. Lower panels show representative 200X micrographs of colonic polyps from APCΔ468, IL-10−/−xAPCΔ468 and CD4CreIL-10fl/flAPCΔ468 mice, showing crypts at the basal surface of the polyp. Inlay images at 400X, basal crypts of the three mouse strains.