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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: Cancer Res. 2013 Aug 16;73(19):5905–5913. doi: 10.1158/0008-5472.CAN-13-1511

Figure 3. Colonic infiltration of eosinophils and mast cells.

Figure 3

(A) Quantification of colon mast cells by CAE (grey bars) and mMCP2 (open bars) for APCΔ468, IL-10−/−xAPCΔ468, and CD4CreIL-10fl/flAPCΔ468 mice.

(B) Representative 400X micrographs of CAE staining (upper panels) or mMCP2 staining (lower panels) of APCΔ468, IL-10−/−xAPCΔ468, and CD4CreIL-10fl/flAPCΔ468 colonic polyps. Arrows point to mast cells. Inlay at 1000X.

(C) Quantification of colon eosinophils by MBP staining in the submucosa (grey bars) and mucosa (open bars) for APCΔ468, IL-10−/−xAPCΔ468, and CD4CreIL-10fl/flAPCΔ468 mice.

(D) Representative 400X micrographs of MBP staining in the submucosa (upper panels) or mucosa (lower panels) in APCΔ468, IL-10−/−xAPCΔ468, and CD4CreIL-10fl/flAPCΔ468 colonic polyps. Arrows point to eosinophils. Inlay at 1000X.

(E) Quantification of eosinophils by MBP staining in CD4CreIL-10fl/flAPCΔ468 mice in the healthy, marginal, and polyp tissue in the colon.

(F) Representative micrographs of CD4CreIL-10fl/flAPCΔ468 polyps stained with the eosinophil-specific marker MBP. Colonic polyps (at 50X) with inlay (at 400X) showing clusters of MBP+ cells.