Table 1.
IC50 of lithocholic acid amphiphiles againt three colon cancer cell lines, HCT-116, DLD-1 and HCT-8.a
| Amphiphile | HCT-116 (μM) | DLD-1 (μM) | HCT-8 (μM) | HPLC, RTb (min) |
|---|---|---|---|---|
| LCA | 81.1 ± 3.8 | 173.1 ± 3.0 | 97.4 ± 2.5 | -c |
| LCA-AMM1 | 36.7 ± 3.4 | 29.4 ± 2.3 | 30.7 ± 6.9 | -d |
| LCA-PPZ1 | 102.0 ± 4.3 | 118.4 ± 4.5 | 114.6 ± 4.1 | -d |
| LCA-MOR1 | 89.0 ± 2.7 | 176.2 ± 3.8 | 120.9 ± 3.4 | 24.17 |
| LCA-TMOR1 | 87.3 ± 3.1 | 163.2 ± 2.7 | 130.5 ± 4.1 | 28.63 |
| LCA-TMA1 | 38.1 ± 2.9 | 53.0 ± 1.0 | 37.0 ± 2.3 | 7.94 |
| LCA-PYRO1 | 17.8 ± 2.4 | 17.0 ± 4.6 | 36.4 ± 2.9 | 8.23 |
| LCA-PIP1 | 11.7 ± 3.0 | 13.7 ± 1.0 | 14.7 ± 3.0 | 8.12 |
| LCA-PYR1 | 26.5 ± 2.0 | 35.4 ± 2.8 | 35.4 ± 4.3 | 7.84 |
| LCA-DMAP1 | 13.2 ± 3.0 | 17.0 ± 2.9 | 59.1 ± 4.5 | 7.90 |
| LCA-DABCO1 | 60.7 ± 1.2 | 46.4 ± 2.9 | 76.4 ± 5.9 | 7.98 |
a
IC50 values were calculated from MTT experiments
b
Mobile phase for all amphiphiles was AcCN:MeOH (95:5) except for LCA-MOR1 and LCA-TMOR1, where mobile phase was AcCN. LCA-MOR1 and LCA-TMOR1 stock solutions were made in 10% EtOAc in AcCN.
c
not determined
d
could not be determined as this amphiphile is soluble in DMSO only.