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. 2015 Feb 10;5:7. doi: 10.3389/fcimb.2015.00007

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Copyright © 2015 Arciola, Campoccia, Ravaioli and Montanaro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double staining with FITC-Wheat Germ Agglutinin (FITC-WGA, for exopolysaccharide detection) and SYPRO Ruby (FilmTracer™ SYPRO® Ruby Biofilm Matrix Stain, for protein detection) was carried out as described in Ravaioli et al. (2012). (A) Green channel image showing PNAG stained with FITC-WGA. (B) Red channel image showing the proteic component stained with SYPRO® Ruby. (C) Merged image of the two channels.