Figure 5.

Western blot for SNAI1, E-cadherin, and vimentin.

Notes: MKN-45 cells that were transfected with an miR-153 control and miR-153, respectively, were subjected to western blot for SNAI1, E-cadherin, and vimentin. Representative western blot showed that upregulation of miR-153 obviously increased protein expression of E-cadherin and reduced SNAI1 and vimentin expression, in gastric cancer cells. SGC-7901 cells that were transfected with miR-153 inhibitor (anti-miR-153) and negative control, respectively, were subjected to western blot for SNAI1, E-cadherin, and vimentin. Representative western blot showed that downregulation of miR-153 obviously decreased protein expression of E-cadherin and increased SNAI1 and vimentin expression, in gastric cancer cells.

Abbreviations: miR-153, microRNA-153; NC, negative control.