Figure 4. Single channel conductance properties of hPiezo1 expressed in the HEK cell line.

(4a).: Original registration from untransfected HEK-293 cell (configuration shown schematically at top). (4b).: same as 4a, but cell was transfected with biscistronic pIRES2 containing hPiezo1 and eGFP as inserts. (4c).: Average I/V relations for single I_Λ inward currents carried by 153 mmole/L K⁺ (grey circles) and 100 mmole/L Ca²⁺ (grey triangles). White symbols denote I_Λ's recorded from MCF-7 cells, lines represent a linear regression through the data (N = 5–8). (4d).: Ion selectivity profile of inward single channel conductance carried by different mono- and divalent cations and by Na^+::K⁺ at a 1:1 ratio. Number of individual experiments is shown at the top of each bar in parenthesis. Mean values ± SEM is shown. (4e).: Quantitative RT-PCR assessing the fold increase in the number of hPiezo1 mRNA transcripts upon transient transfection of HEK-293 cells with the pIRES2 construct (data were derived from 3 individual transfection experiments). Mean values ± SEM are shown.