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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: Science. 2008 Apr 11;320(5873):226–230. doi: 10.1126/science.1154986

Fig. 2.

Fig. 2

CBLB502-mediated protection of radiosensitive tissues (18). (A) Representative terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining of apoptotic cells (5 hours postirradiation) in the small intestine of NIH-Swiss mice injected with CBLB502 or PBS 30 min before 15 Gy TBI. Apoptotic endothelial cells displayed yellow fluorescence resulting from overlap of green TUNEL staining and red CD31-specific antibody (endothelial marker) staining. Nuclei were stained with 4′,6′-diamidino-2-phenylindole (DAPI, blue). (B) Morphology of the small intestine in NIH-Swiss and MOLF/Ei mice either untreated or 5 days after 15 Gy TBI with or without prior injection of CBLB502. Representative hematoxylin and eosin (H&E) stained sections are shown. (C) Immunohistochemical detection of in vivo BrdU incorporation in the crypts of the small intestine. Mice (three per group) were left untreated (U/t), given CBLB502 without TBI (CBLB502), or exposed to 13 Gy TBI 30 min after injection of PBS (13Gy)orCBLB502(13Gy+CBLB502). BrdU-positive cells were counted in 12 complete, well-oriented cross sections for each animal. Dashed line: number of BrdU-positive cells considered critical for crypt survival (36). P < 0.05 for the differences between “13 Gy” and other groups. (D) Granulocyte-macrophage colony-forming units were quantified in bone marrow cells obtained from NIH-Swiss mice (n = 3) 3 hours after 0, 10, or 13 Gy TBI (with or without CBLB502 injection 30 min before TBI). P < 0.05 for the differences between irradiated CBLB502- and vehicle-treated groups. (E) Immunohistochemical detection of SOD2 expression (green staining) in sections of small intestine collected 5 hours post-irradiation from NIH-Swiss mice injected with either PBS (13 Gy) or CBLB502 (13Gy + CBLB502) 30 min before 13 Gy TBI. Red staining, smooth muscle actin. (F) Cytokine induction by CBLB502 treatment in the absence of irradiation. Plasma was prepared at the indicated times (0.5 to 24 hours) after intramuscular injection of CBLB502 into ICR mice (n = 3). KC, keratinocyte-derived chemokine; IP-10, interferon-inducible protein 10.