Figure 2.
Mitochondrial respiration is inefficient in insulin-resistant UCP-DTA hearts. A, Mitochondrial respiration rates (V̇o) in saponin-permeabilized muscle strips prepared from NTG and UCP-DTA hearts in the presence of palmitoyl-l-carnitine/malate. Mean values (±SE) are shown for basal, state 3 (ADP-stimulated), and oligomycin-inhibited (oligo) respiration. The respiratory control (RC) ratio represents the ratio of oligo to state 3 respiration; n=6 animals/group. B, ATP synthesis rates (left) and ratio of ATP synthesis to maximal V̇o (ATP/O; right) obtained from the same muscle strips shown in Figure 2A. *P<0.05 compared with NTG value. mg dw indicate milligrams of dry weight.