Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Arch Biochem Biophys. 2014 Dec 19;567:66–74. doi: 10.1016/j.abb.2014.12.014

Fig. 3.

Fig. 3

Inhibition of Bzm-ShhN induced AP and transcriptional activity in C3H10T1/2 cells. (A) C3H10T1/2 cells were incubated with Shh proteins at EC50 values (2 μg/ml for unmodified ShhN, 0.02 μg/ml for ShhNp and Bzm-ShhN) for 5 days and induction of GLI and Ptch1 mRNA measured by RT-PCR relative to β–actin. (B) C3H10T1/2 cells were incubated with Shh proteins at EC50 values (2 μg/ml for unmodified ShhN, 0.02 μg/ml for ShhNp and Bzm-ShhN) for 5 days with and without 300 nM KAAD-cyclopamine (KAAD-cyc) and induction of AP activity measured as before. (C) Inhibition of ShhN induced GLI1 transcription activity in C3H10T1/2 cells. Shh proteins were incubated at EC50 values (2 μg/ml for unmodified ShhN, 0.02 μg/ml for ShhNp and Bzm-ShhN) for 5 days with and without 1 μM KAAD-cyclopamine. GLI1 mRNA expression was measured by RT-PCR relative to β–actin.