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Dual immunofluorescence for γ-tubulin and Polo-like kinase 1 (PLK1) (A &B), Aurora-kinase A (AURKA) (C & D) and PCNT (E & F). Interphase cells reveal co-localization of γ-tubulin with PLK1 (A) and PCNT (E) but not with AURKA (C). In mitosis, however, all four protein co-localize (D & E), however PLK1 remains at the metaphase plate during anaphase (B) and is found as two rings at the midbody after cytokinesis.
