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. 2015 Jan 21;9:23. doi: 10.1186/1944-3277-9-23

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Copyright © 2015 Nielsen et al.; licensee BioMed Central.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Consumption of oxygen (□; measured online with an oxygen microsensor) and nitrate (●; measured by HPLC) during growth (▲; OD₆₀₀) of B. azotoformans MEV2011. No growth was observed at oxygen concentrations >30-35 μM, and the initiation of growth coincided with the first detection of ³⁰ N₂ from ¹⁵NO₃⁻ (data not shown), indicating that growth was coupled to denitrification.