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. 2014 Nov 3;5(23):12126–12140. doi: 10.18632/oncotarget.2573

Figure 3. CYLD ablation results in increased Notch signalling.

Figure 3

(A) HEK-293T cells stably expressing a RBP-JK response element coupled to a firefly luciferase were used as a readout for active Notch signalling. Notch signalling was significantly increased following CYLD knockdown with siRNA targeting CYLD (unpaired t-test). (B) CYLD protein expression reduction following siRNA targeting was demonstrated by probing lysates from transfected cells with anti-CYLD antibody (C) CYLD protein expression reduction is associated with increased JAG2 expression, and (D) increased JAG2 expression is seen in the majority of CYLD defective tumour lysates obtained from patients with germline mutations in CYLD.