Figure 5. Expression of Notch target genes in CYLD defective tumours.

(A) Tissue microarrays of cylindromas and spiradenomas and perilesional control skin were probed with anti-RUNX1, anti-LEF1 and anti–HES1 antibody and protein expression was visualized with a horseradish peroxidase/3,3′-Diaminobenzidine (DAB) system, with haematoxylin used as a nuclear counterstain. Nuclear staining of LEF1, RUNX1 and HES1 was seen in CYLD defective tumours. Scale bars indicate 100μm. (B) Notch target proteins are increased in CYLD defective tumours when compared to perilesional control skin. Photomicrographs of tumour sections and control tissue were subject to quantitation of DAB staining as a readout of protein expression. This demonstrated an increase in expression of LEF1 ( p = 0.001), RUNX1 ( p = 0.013) and HES1 ( p = 0.027) in CYLD defective tumours when compared to control tissue. All p-values indicated were derived using a t-test. Error bars indicate standard error of the mean