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. 2014 Nov 3;5(23):12126–12140. doi: 10.18632/oncotarget.2573

Figure 6. Primary cultures of CYLD defective tumours are sensitive to γ-secretase inhibitors.

Figure 6

Primary cells (CPCC) derived from fresh tumour tissue obtained following surgical procedures on CYLD mutation carriers or a keratinocyte cell line (HACAT) were cultured on 3D tissue culture scaffolds. After 28 days, scaffolds were grown in the presence of two different γ-secretase inhibitors (YO-0107 and LY 450139) for 12 days in triplicate and cell viability was assessed using an ATP dependent luminescent assay (Cell-Titre Glo – Promega UK). This was repeated using at least three different tumours, from different body sites in three patients. Data displayed represents the mean of 4 biological replicates of CPCC, with 3 technical replicates each. Cylindroma cells demonstrated differential sensitivity to inhibition with γ-secretase inhibitors at low micromolar concentrations of YO-0107 and LY 450139. Error bars indicate standard error of the mean. p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***) as indicated, calculated using a t-test.