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. 2014 Nov 28;5(23):11778–11791. doi: 10.18632/oncotarget.2634

Figure 1. MSP RAS cells resist chemotherapeutic treatment and apoptosis induction.

Figure 1

(A) HMLE RAS cells were treated with 5μM or 10μM cisplatin, for 24h, followed by further incubation without chemotherapeutics. The cell number was determined daily for 7d, using automated light microscopy with quantitative image analysis (Celigo cytometer). Non-treated cells were used as controls. (B-F) HMLE RAS cells were treated with the indicated concentrations of cisplatin (B) or carboplatin (C), each for 16h, with doxorubicin for 24h (D), with neocarzinostatin for 20h (E) or Trail in the presence of 20μg/ml cycloheximide for 6h (F). Controls were treated with corresponding amounts of the respective solvent. Cell lysates were analysed by immunoblotting. β-Actin or HSC70 staining was used as loading control.