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. 2014 Nov 28;5(23):11778–11791. doi: 10.18632/oncotarget.2634

Figure 5. The BH3-mimetics gossypol and Abt-737 overcome apoptosis resistance of MSP RAS cells.

Figure 5

(A, B) HMLE RAS cells were treated with gossypol (A) or Abt-737 (B) for 24h, followed by further incubation without the drug. DMSO was used as control. Cell number or cell confluence was measured daily for 8d and 7d with a Celigo cytometer, respectively. (C) HMLE RAS cells were treated with 10μM Gossypol or 10μM Abt-737 for 48h and 72h, respectively. Cell viability upon treatment was determined by assessing the ATP concentration in cell lysates using a luciferase assay. (D) HMLE RAS cells were exposed to gossypol, or DMSO, in the presence or absence of 20μM cisplatin, for 16h. (E) HMLE RAS cells were treated with Abt-737 for 16h. Cell lysates were analysed by immunoblotting. LC3a/b II served as an indicator for autophagy. During autophagy, the cytosolic LC3a/b I (16kDa) is converted to the autophagosome-associated LC3a/b II (14kDa) through lipidation [27].