Figure 6. BH3-mimetics prolong the survival of mice carrying MSP RAS xenografts.
(A) 106 MSP RAS cells were subcutaneously injected in (Nu/Nu) mice to form a tumor xenograft. Three weeks thereafter, the mice were treated with intraperitoneal injections of 75mg/kg/day Abt-737 (light grey line, n = 10) or vehicle (dark line, n = 9) for 21d. The mice were euthanized when a tumor size of 15mm in diameter had been reached. The shaded area corresponds to the duration of treatment. (B) IHC and IF of xenograft tumor tissue for detection of Ki67 or LC3, respectively, in control and Abt-737 treated mice. 600x magnification for LC3, inlet 4x zoom. 100x magnification for Ki67. A more extensive array of stainings is shown in Supplemental Fig. S6. (C) Quantification of LC3 positive cells across all treated vs. mock-treated tumors. (D) Quantification of Ki67 positive cells. Columns and error bars represent the mean ± S.E.M.