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. 2014 Nov 13;5(23):11909–11923. doi: 10.18632/oncotarget.2557

Figure 2. Overexpression of RFPL3 up-regulates hTERT promoter acivation and promotes hTERT expression and telomerase activity.

Figure 2

(A) WI-38, HBE and A549 cells were co-transfected with hTERT-luciferase and the indicated doses of pCDNA3.1 empty vector or pCDNA3.1-RFPL3 plasmid for 48 h. Luciferase activity was measured using a dual-luciferase assay. The activation of luciferase was calculated relative to cells transfected with empty vector. All of the measurements represent the means ± SE of three independent experiments (*P < 0.05). (B) WI-38, HBE and A549 cells were transfected with pCDNA3.1 empty vector or pCDNA3.1-RFPL3 plasmid for 48 h, and the expression of hTERT mRNA and protein was analyzed by RT-PCR and Western blot. (C)Telomerase activity was measured in WI-38, HBE and A549 cells using a telomeric repeat amplification protocol assay-based TeloTAGGG telomerase PCR enzyme-linked immunosorbent assay (**P < 0.01).