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. 2014 Nov 13;5(23):11909–11923. doi: 10.18632/oncotarget.2557

Figure 3. Knockdown of RFPL3 inhibits hTERT promoter activation and decreases hTERT expression and telomerase activity.

Figure 3

(A) The A549 and H1299 cells were co-transfected with the hTERT-luciferase plasmids and the indicated doses of RFPL3 siRNA or a non-specific control siRNA for 48 h. Luciferase activity was measured using a dual-luciferase assay. The inhibition of luciferase was calculated as a percentage relative to cells transfected with control siRNA. All of the measurements represent the means ± SE of three independent experiments (*P < 0.05). (B) The A549 and H1299 cells were transfected with RFPL3 siRNA or non-specific control siRNA for 48 h, and the expression of hTERT mRNA and protein was analyzed by RT-PCR and Western blot. (C) Telomerase activity was measured in A549 and H1299 cells by telomerase PCR enzyme-linked immunesorbent assay (**P < 0.01).