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. 2014 Oct 21;5(23):12111–12125. doi: 10.18632/oncotarget.2545

Figure 3. Occupancy analysis establishes ETV7 and LAMP3 as direct p65 and/or p53 target genes.

Figure 3

(A) Relative quantification of immune-precipitated gene fractions by qPCR from MCF7 cells subjected to Doxo or TNF⍺ single treatments and to the double treatment. The antibodies used for the immune-precipitations are listed. P21 was used as positive control, while ACTB was used as a negative control. Plotted are the average percentages relative to input signals. Error bars represent the standard errors of at least three biological replicates. (B) as in A, but probing p65 occupancy. MCP1 was used as positive control. The IgG antibody controls were anti-mouse (A) or anti-rabbit (B) to match the specific primary antibodies. (C) The position of the primers used for the qPCR and the location of predicted p53 and p65 binding sites in the ETV7 and LAMP3 genes are depicted.