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. 2014 Nov 4;5(23):12410–12417. doi: 10.18632/oncotarget.2678

Fig.1. Alcohol induces RNA Pol III-dependent transcription.

Fig.1

ER+ breast cancer cell lines (MCF-7 and T47D), ER- breast cancer cell lines (MDA-MB231 and SKBR-3) and ER- non tumor cell lines (MCF-10F, MCF-12A and MCF-10A) were starved in FBS/DMEM-F12 for 3h. Cells were treated with or without 25 mM of ethanol. RNAs were isolated from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (A), 5S rRNA (B). The fold change was calculated by normalizing to the amount of GAPDH mRNA. The bars represent Mean ± SE of at least three independent determinations.