Fig.2. Tam represses RNA Pol III-dependent transcription.
(A and B): the cells of ER+ MCF-7 breast cancer lines were starved in DMEM-F12 for 3h. Cells were pretreated with different amounts of Tam for 1 hour, and then treated with 25 mM ethanol for another 1 h as indicated in Fig. 1. (C-F): the cells of ER- MDA-MB231 (C and D) and SKBR-3 (E and F) were pretreated with 12.5 μM Tam for 1 h and then treated with 25 mM ethanol. Total RNAs were extracted from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (A, C, E) and 5S rRNA (B, D, F). The fold change was calculated by normalizing to the amount of GAPDH mRNA. The bars represent Mean ± SE of at least three independent determinations.