Fig.5. Tam reduces ERα binding to the promoters of Brf1.
(A-C) ERα binding to Brf1 promoter. Schematic of the Brf1 promoter and primers used for ChIP assays are designated relative to the ERα site (A). MCF-7 cells treated with Tam and alcohol as described above to extract chromatin. ChIP assay were performed with ERα antibody to determine occupancy of ERα in the Brf1 promoter; (D-E) Brf1 binding to the promoters of Pol III genes The cells were treated as described above. ChIP assay were performed with Brf1 antibody and qPCR to quantify the amplified DNA of tRNALeu (D) and 5S rRNA (E). The relative occupancy of the proteins was calculated based on the control (no Tam treatment). All values shown are the means ± SE of at least three independent experiments.