Table 5. Concordance of C228T mutation between tumor tissues and urine samples as determined using Sanger sequencing and castPCR.

	Tumor tissues
UTUC	Sanger	Mutant	WT	Sensitivity	Specificity	Accuracy
Urine samples	Mutant	6	1	60% (26 – 88%*)	97% (86 – 100%)	89%
	WT	4	36
	Total	10	37

	castPCR	Mutant	WT	Sensitivity	Specificity	Accuracy
Urine samples	Mutant	9	3	90%(56 – 100%)	92% (78 – 98%)	94%
	WT	1	34
	Total	10	37

	Tumor tissues
BC	Sanger	Mutant	WT	Sensitivity	Specificity	Accuracy
Urine samples	Mutant	17	0	47% (30 – 65%)	100% (89 –100%)	73%
	WT	19	33
	Total	36	33

	castPCR	Mutant	WT	Sensitivity	Specificity	Accuracy
Urine samples	Mutant	31	1	86% (67 – 94%)	97% (82 – 100%)	90%
	WT	5	32
	Total	36	33

	Tumor tissues
UTUC + BC	Sanger	Mutant	WT	Sensitivity	Specificity	Accuracy
Urine samples	Mutant	23	1	50% (35 – 65%)	98% (92 – 100%)	76%
	WT	23	69
	Total	46	70

	castPCR	Mutant	WT	Sensitivity	Specificity	Accuracy
Urine samples	Mutant	40	3	89% (74 – 96%)	96% (88 – 99%)	91%
	WT	6	67
	Total	46	70

McNemar's Test for differences between Sanger sequencing and castPCR assays

Sensitivity: UTUC, P = 0.37; BC, P = 0.003; UTUC +BC: P < 0.001.

Specificity: UTUC, BC and UTUC + BC: P ≥ 0.48.

*95% confidence intervals.