Figure 1. AGK is upregulated in HCC cell lines and primary human HCC tissues.

(A and B) Western blotting analysis of AGK expression in two immortalized normal liver cell lines and 11 HCC cell lines (A) and in eight paired primary HCC tissues (T) and the adjacent noncancerous tissues (ANT) from the same patients (B); α-tubulin was used as a loading control. (C) IHC staining of AGK expression in human HCC (clinical stages I–IV) and normal liver tissues. (D) Kaplan-Meier overall survival curves for patients with HCC stratified by low (n = 124) and high (n = 121) expression of AGK (P < 0.001). (E) GEO data (GSE25097) analysis of AGK mRNA expression in normal liver tissues from healthy individuals (n = 6), cirrhotic tissues (n = 40) and HCC tissues (n = 256); **P < 0.001.