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. 2014 Oct 21;5(23):11857–11872. doi: 10.18632/oncotarget.2613

Figure 4. Hydroxylation of HIF-1α drives dissociation of HIF-1α-VHL complex from PLD1.

Figure 4

(A) Effect of VHL on the interaction of PLD1 with HIF-1α in the presence ofMG132. The band intensity was quantified. Data are representative of three independent experiments. (B) Effect of PHD2 on the interaction of VHL with PLD1. Data are representative of three independent experiments.(C) Effect of PHD2 on the interaction of PLD1 with HIF-1α in the presence of MG132. The band intensity was quantified. Data are representative of three independent experiments. (D) Effect of VHL on the interaction of PLD1 with HIF-1α-PPAA. The band intensity was quantified. Data are representative of three independent experiments. (E) IP assay to test the effects of mutants of VHL Y98N and Y112N on theinteraction of PLD1 with HIF-1α in the presence of MG132. The band intensity was quantified. Data are representative of three independent experiments. (F) IP assay to test the effects of hydroxylated HIF-1α peptide (residues 556-574) on the interaction of VHL with HIF-1α and PLD1 in the presence of MG132. ODD-OH, hydroxylated-ODD peptide; ODD, nonhydroxylated-ODD peptide. The band intensity was quantified. Data arerepresentative of three independent experiments.