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. 2014 Dec 5;5(23):11847–11856. doi: 10.18632/oncotarget.2663

Figure 2. Effects of cetuximab on intracellular signaling and the expression of apoptosis-related proteins in DiFi isogenic cell lines.

Figure 2

DiFi-Mock1, DiFi-HRG4, DiFi-HRG5, or DiFi-HRG6 cells were cultured overnight in medium containing 10% serum and then incubated for 6 h (A) or 24 h (B) in serum-free medium with or without cetuximab (10 μg/mL), after which cell lysates were prepared and subjected to immunoblot analysis with antibodies to phosphorylated (p) or total forms of the indicated proteins (left panels). A band corresponding to the cleaved (cl) form of PARP is indicated. The intensity of the bands corresponding to phosphorylated forms of EGFR, HER2, HER3, AKT, and ERK (A) or to BIM and survivin (B) was normalized by that of the corresponding total proteins or β-actin, respectively, and then expressed relative to the corresponding value for control cells not exposed to cetuximab (right panels).