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. 2014 Nov 7;5(23):12141–12150. doi: 10.18632/oncotarget.2559

Figure 2. miR-520d-5p targets TWIST1 in its 3′UTR.

Figure 2

(A) Schematic representation of the predicted binding site of miR-520d-5p in the 3′UTR of TWIST1 (upper) and of the mutated site with seed deletion (TWIST1 Mut, lower). The nucleotide positions are indicated (B) qRT-PCR for the expression of miR-520d-5p in HeLa cells transduced with either a control miR (CTRL) or miR-520d-5p. (C) Control 3′UTR, WT TWIST1 3′UTR or mutated TWIST1 3′UTR (Mut) were fused to firefly luciferase, and co-transfected with renilla luciferase into HeLa cells overexpressing miR-520d-5p. The luciferase activity was measured and normalized to renilla activity. The control 3′UTR activity was set to 1. Results are representative of two independent experiments. **P < 0.015.