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. 2015 Feb 5;13:4. doi: 10.1186/s12953-014-0061-2

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© Ahn et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparative interactions of Prdx V WT or cysteine mutants with DBT in normoxic and hypoxic cells. (A) All WCE presented for every Prdx V mutant showed the same background of cellular expression for each transfected construct. (B) Coprecipitation of HA-tagged Prdx V with myc-tagged DBT. (C) Reverse immunoprecipitation of myc-tagged DBT with HA-tagged Prdx V. HEK293 cells were cotransfected with the HA-tagged Prdx V WT or cysteine mutant (C48S, C73S, C152S, C48/152S) and myc-tagged DBT expression vector, and then incubated under normoxic and hypoxic conditions. WCE, whole cell lysate of HEK293 cell; Mock, the empty vector tagged to myc or HA.