Table 1.
Protein showing altered interaction by Prdx V immunoprecipitation during hypoxic stress a
| Accession | Description | Gene | Score | MS/MS spectra | Frequency b | |
|---|---|---|---|---|---|---|
| Normoxia | Hypoxia | |||||
| IPI00130467 | Ras related protein Rab 43 isoform b | Rab43 | 228.7 | 6 | ND | 1/3 |
| IPI00130535 | Lipoamide acyltransferase component of branched chain α-keto acid dehydrogenase complex, mitochondial | Dbt | 302.6 | 8 | ND | 2/3 |
| IPI00131695 | Serum albumin | Alb | 325.3 | 3 | 1/3 | 3/3 |
| IPI00225123 | Non-lysosomal glucosylceramidase | Gba2 | 191.4 | 9 | 1/3 | ND |
| IPI00226993 | Thioredoxin | Txn1 | 1155.1 | 3 | 3/3 | 2/3 |
| IPI00330523 | Propionyl CoA carboxylase alpha chain, mitochondrial | Pcca | 474.0 | 21 | 2/3 | 3/3 |
| IPI00346834 | Keratin type II cytoskeletal 2, oral | Krt76 | 184.2 | 7 | ND | 1/3 |
| IPI00348328 | Keratin Kb40 | Krt78 | 580.5 | 19 | 1/3 | ND |
| IPI00122281 | Keratin type I cuticular Ha2 | Krt32 | 260.3 | 8 | 1/3 | ND |
| IPI00227140 | Keratin type I cytoskeletal 14 | Krt14 | 1399.7 | 16 | ND | 1/3 |
| IPI00230365 | Keratin type I cytoskeletal 17 | Krt17 | 1322.1 | 16 | ND | 1/3 |
| IPI00347019 | Keratin type II cuticular Hb4 | Krt84 | 330.8 | 10 | ND | 1/3 |
| IPI00347096 | Keratin type II cytoskeletal 72 | Krt72 | 337.2 | 8 | ND | 1/3 |
| IPI00420970 | Keratin type II cytoskeletal 74 | Krt74 | 1999.7 | 4 | 1/3 | 3/3 |
| IPI00462140 | Keratin type II cytoskeletal 1b | Krt77 | 2014.2 | 7 | ND | 1/3 |
| IPI00468696 | Keratin type I cytoskeletal 42 | Krt42 | 798.6 | 12 | ND | 1/3 |
| IPI00606510 | Propionyl CoA carboxylase beta chain, mitochondrial | Pccb | 266.6 | 10 | 2/3 | 3/3 |
aProteins were affinity-purified from mouse kidneys under both normoxic and hypoxic conditions as bound interactors with Prdx V immunoprecipitation. The purified immunoprecipitates were applied to acrylamide gel-associated tryptic digestion and subjected to nano-UPLC-MS/MS for protein identification.
bFrequency represents the number of times that the interactors were observed in three independent experiments. ND, not detected.