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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: J Biol Chem. 2005 May 27;280(29):27375–27382. doi: 10.1074/jbc.M503693200

FIG 4. Bβ2 interacts with TOM22 in intact cells.

FIG 4

A, schematic of fusion proteins used for luciferase complementation. B, model of PP2A/ Bβ2 interaction with the TOM translocase complex. C- and N-terminal fragments of luciferase (NLuc and CLuc) reconstitute an active enzyme when their fusion partners Bβ2 and TOM22 interact. C, complementation between CLuc-Aα and Bβ2-NLuc in intact, transiently transfected COS-M6 cells is unaffected by the K2A mutation. D, binding to TOM22 requires Lys2 of Bβ2 (significant difference, p > 0.001 by Student's t test). The bar graphs show average luciferase activities ± S.E. from triplicate cotransfections of the indicated fusion proteins. C and D, the immunoblots with a pan-B subunit antibody were performed on cells lysed after the luciferase assays and show equal expression levels of wild-type and mutant Bβ2-NLuc. The position of the 97-kDa marker is shown.