Figure 5.

Inhibition of NO and PGE ₂ production by SSeo in TNF-α-treated HASMCs. HASMCs were pretreated with various concentrations of SSeo for 1 h before incubation with TNF-α (100 ng/ml) for 24 h. (A) Nitrite content was measured using the Griess reagent and (B) PGE₂ concentration was measured in culture media using a commercial ELISA kit. Values represent the mean ± SD of three independent experiments. We assessed differences between mean values by the Student’s t-test. *p < 0.05 indicates significant differences from the TNF-α-treated group.