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Effects of SSeo on TNF-α-induced intracellular ROS generation in HASMCs. HASMCs were treated with NAC (10 mM) or the indicated concentrations of SSeo for 30 min before treatment with TNF-α (100 ng/ml) for 30 min. The cells were incubated with 20 mM H2DCFDA at 37°C for 30 min, and ROS generation was measured using a flow cytometer. Each point represents the mean of two independent experiments.
