Figure 8.

Inhibition of NF-κB nuclear translocation by SSeo in TNF-α-stimulated HASMCs. (A) Cells were pretreated with SSeo (20 μg/ml) for 1 h before TNF-α treatment (100 ng/ml) for the indicated times. (A) Cytosolic and nuclear proteins were run on 10% SDS-polyacrylamide gels followed by Western blotting using anti-NF-κB p65 and anti-IκB-α antibodies, and an ECL detection system. Nucleolin and actin were used as internal controls for the nuclear and cytosolic fractions, respectively. (B) The cells were pretreated with SSeo (20 μg/ml) for 1 h before TNF-α treatment (100 ng/ml). After a 1 h incubation, localization of NF-κB p65 was visualized with fluorescence microscopy after immunofluorescence staining with anti-NF-κB p65 antibody (green). Cells were also stained with DAPI to visualize nuclei (blue). Results are representative of those obtained from three independent experiments.