Figure 1.

Phosphorylation of β ₃ Tyr773 is required for VEGF ₁₆₅ -induced cell migration and cell surface NCL localization. (A) Protein extracts of CHO cells were analysed for expression of VEGFR2. HUVEC were used as a positive control and β-actin as a loading control. (B) Effect of VEGF₁₆₅ (10 ng/ml) on CHO cell migration. Data are from five independent experiments and are expressed as mean ± s.e.m. percentage change in number of migrating cells compared with the corresponding non stimulated cells (set as default 100). (C) Immunofluorescence images stained for NCL (green) and nucleus (blue) in serum starved CHO cells treated with VEGF₁₆₅ (10 ng/ml) for 5 h at 37°C. Vector, cells transfected with the plasmid vector; wtβ₃, cells over-expressing wild-type β₃; β₃Y773F, cells over-expressing β₃Y773F; β₃Y785F, cells over-expressing β₃Y785F; β₃Y773F/Y785F, cells over-expressing double mutant β₃Y773F/Y785F.