Figure 6.

Effect of CS-E on VEGF ₁₆₅ -induced endothelial cell signaling and migration. (A) Formation of VEGF-RPTPβ/ζ complexes as evidenced by in situ PLA in HUVEC in the absence or the presence of CS-E II (100 ng/ml). The box plots indicate the median, mean and range of the detected signals (n > 20 image fields with ~4 cells per image per sample type, each sample run at least in duplicate) from three independent experiments. (B) Immunofluorescence images stained for NCL (green) and nucleus (blue) in serum starved HUVEC treated for 5 h at 37°C with VEGF₁₆₅ (10 ng/ml) in the absence or the presence of CS-E II (100 ng/ml). Representative pictures from two independent experiments. (C) Effect of CS-E I and II (both at 100 ng/ml) on VEGF₁₆₅-induced HUVEC migration. Data are from three independent experiments and are expressed as mean ± s.e.m. percentage change in number of migrating cells compared with the non stimulated untransfected cells (set as default 100). (D) Immunofluorescence images stained for NCL (green) and nucleus (blue) in serum starved HUVEC treated for 5 h at 37°C with VEGF₁₂₁ (10 ng/ml). Representative pictures from two independent experiments. Scale bars in all cases correspond to 10 μm.