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. 2015 Feb 3;15:27. doi: 10.1186/s12870-015-0426-4

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© Cao et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of ROS levels and the activities of related enzymes in calli. M-33, and RB-4 represent the ECM lines of Marsh grapefruit and Star Ruby grapefruit, respectively. WT represents wild-type calli. (A) O₂ ^- is detected by histochemical staining with NBT. M and RB are shown as representatives for O₂ ^- levels. (B) H₂O₂ determination is based on the titanium reagent method. (C) NOX activity (nmol NADPH/min/g FW), SOD activity (U/g FW) and CAT activity (U/g FW) were analyzed in the M-33 and its wild-type control. The columns and bars represent the means and ± SD, respectively (n = 3 replicate experiments). **Indicates that the values are significantly different compared with wild type at the significance level of P < 0.01.