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. 2015 Feb 5;15:39. doi: 10.1186/s12870-015-0431-7

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© Chuang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evolutionary analysis of the newly-identified exons and their flanking exons. (A) Comparisons of evolutionary rates (dn, ds, and dn/ds) between the newly-identified exons and their flanking exons. Statistical significance was estimated by the paired two-tailed Wilcoxon signed rank-sum test. **P < 0.01 and ***P < 0.001. Error bars represent the standard errors of the means. (B) Proportions of newly-identified ASVs with and without significant differences in evolutionary rates between the novel exons and their flanking exons (P < 0.05 by the two-tailed Fisher’s exact test; Methods). Novel_dn and Novel_ds represent the dn and ds values of the novel exons; Flanking_dn and Flanking_ds represent the dn and ds values of their flanking exons, respectively.