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. 2015 Feb 5;15:39. doi: 10.1186/s12870-015-0431-7

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© Chuang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Experimental validations of the newly-identified exons/ASVs. Shown in the figure are. RT-PCR products of the newly-identified isoforms that include the novel exons and the previously-annotated isoforms that exclude the novel exons in (A) O. sativa L. ssp. japonica cv. Nipponbare (designated as “Nip”) and (B) O. sativa L. ssp. indica cv. 93-11 (designated as “93-11”), O. rufipogon (designated as “Ruf”), O. nivara (designated as “Niv”), and O. glaberrima (designated as “Gla”). The black and gray arrows represent the newly-identified and previously-annotated isoforms, respectively.