Table 2.

Variation of melting temperature and cycle threshold in robustness tests for qPCR.

Species	Sample	Robustness 1*		Robustness 2
		Ct	Melting	Ct	Melting
M. tuberculosis	1	12.95	94.4	19.44	94.1
	2	18.31	94.4	29.44	94.5
	3	16.30	94.5	26.73	94.4
	4	17.37	93.56	24.88	93.63
	5	19.36	94.43	31.76	93.6
M. bovis	1	—-	—-	—-	—-
	2	—-	—-	—-	—-
	3	—-	—-	—-	—-
	4	—-	—-	—-	—-
	5	—-	—-	—-	—-

^*Robustness 1: increase in 1 °C in annealing temperature and 10% less enzyme, MgCl₂ and primers; Robustness 2: decrease by 1 °C temperature annealing and 10% more enzyme, MgCl₂ and primers.