Table 5.
Substrate* | Enzyme production (IU/mL) | |||||
---|---|---|---|---|---|---|
|
||||||
Amylase | Xylanase | Pectinase# | Endoglucanase | β-glucosidase | Filterpaperase | |
Control | 0.786 ± 0.46 | 0.29 ± 0.148 | 5.15 ± 0.138 | 0.6906 ± 0.348 | 0.246 ± 0.311 | 0.766 ± 0.473 |
Xylan | 0.186 ± 0.166 | 0.24 ± 0.175 | 0.28 ± 0.03 | 0.273 ± 0.168 | 0.42 ± 0.384 | 0.394 ± 0.106 |
Pectin | 0.0713 ± 0.044 | 0.1 ± 0.085 | 0.12 ± 0.102 | 0.146 ± 0.084 | 0.0493 ± 0.024 | 0.073 ± 0.044 |
Starch | 0.098 ± 0.103 | 0.307 ± 0.153 | 0.05 ± 0.046 | 0.114 ± 0.127 | 0.172 ± 0.086 | 0.4126 ± 0.211 |
CMC | 0.1046 ± 0.05 | 0.333 ± 0.168 | 0.1 ± 0.014 | 0.48 ± 0.272 | 0.46 ± 0.348 | 1.2 ± 0.633 |
[Data represent the average of three independent experiments; each enzyme productivity was observed in triplicate ± SD].
The substrates were added at 0.5% xylan, 1% pectin, 1% CMC and 0.5% starch separately.
The actual values of pectin will be obtained after multiplying it with 15.