Table 2. Yields of Oligonucleotides at Various Stages of Deprotection. Characterization Using MALDI-TOF Mass Analysis Is Shown for Oligonucleotides That Were Purified.

^*Subscript 2′-OAc indicates 3′,5′-linkage with 2′-OAc protection at the modification site while 3′-OAc indicates 2′,5′-linkage with 3′-OAc.

^aThis oligonucleotide was synthesized using commercial amidites and was deprotected with 3:1 saturated ammonium hydroxide/EtOH.

^bThe overall synthesis yield was obtained by comparing the total released amount of oligonucleotide from CPG to the synthesis cycle scale (1 μmol).

^cThe cleavage yield was obtained by comparing the cleaved amount of oligonucleotide to the final trityl yield.