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Co-culture systems used in the experiments. A. Hepatocyte monoculture. HepLi5 cells were inoculated into the lower layer of a Transwell dish. B. Mixed co-culture. HepLi5 and DiI-HSC-Li cells were all inoculated into the lower layer of a Transwell dish. C. Separated co-culture. HepLi5 and DiI-HSC-Li cells were inoculated into the lower and upper layers of a Transwell dish, respectively.
