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. 2015 Feb 8;12(3):248–255. doi: 10.7150/ijms.11002

Figure 7.

Figure 7

The effects of co-culturing with HSC-Li cells on hepatocyte-specific functions of HepLi5 cells. A-D. The ratio of gene expression levels in HepLi5 cells between monolayer culturing and co-culturing as determined using real-time RT-PCR. The relative expression levels were determined by the Delta-Delta Ct method and normalized to GAPDH. The mRNA expression levels of albumin, CYP3A5, CYP2E1, and UGT2B7 in HepLi5 cells in mixed co-culture and in separated co-culture were generally higher than the levels in monolayer-cultured HepLi5 cells alone. (E). The CYP1A2 activity of HepLi5 cells in separated co-culture and in mixed co-culture was generally higher than in a monoculture of HepLi5 cells.